dsRNA effect on adult neural stem cells

[TairaKawasaki]

How do you think dsRNA effect on adult neuron

We have found a strange coincide in Fig.4 on
Cell. 2004 Mar 19;116(6):779-93.
MID:15035981

Please check it by your own eyes.

[LilKim]

hmm... i don't know the conditions or background for this figure

( and I don't have time to pub med it... at the moment.)

Do you know if this is a RNAi response?

[cartener]

haha, good!
I have a question, who can answer me:
what is the definition of noncoding RNA? is that one do not have the genetic code ATG or this RNA display it.s function at the RNA lever?

[TairaKawasaki]

An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease

[TairaKawasaki]

In 2005, among many other cases, a prominent Japanese scientist who published two major papers in Nature (H. Kawasaki and K. Taira Nature 423, 838?842; 2003 and Nature 431, 211?217; 2004) could not produce the corresponding experimental data. The first paper had already been retracted (Nature 426, 100; 2003) and the second corrected (Nature 431, 878; 2004). An investigation panel asked Taira to submit samples and notebooks relating to the experiments, but the researcher in his lab who ran the experiments did not have them. It is obvious that references to those papers will appear, be it only to state that they are likely to be faked, but this will obviously increase the Impact Factor of the journal... In the same way we can see in october 2005: Retraction: RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription
Vera Schramke, Daniel M. Sheedy, Ahmet M. Denli, Carolina Bonila, Karl Ekwall, Gregory J. Hannon and Robin C. Allshire
Nature 435, 1275–1279 (2005)

In spring 2006, investigation about RNA work in Taira's group (at least 12 papers published in quite "visible" journals are probably fakes).

[LilKim]

hmm??? where did yo hear that? Is it on the nature website? I'm really curious because I read that paper recently. "induction of DNA menthlyation and gene silencing by short interfering RNA's in human cells" by kawasaki and taira

[LilKim]

never mind.. I found the reference thanks!

[TairaKawasaki]

All the 12 papers indicated in natutre are written by Dr. Kawasaki, an assistant researcher working for Dr. Taira at Univ of Tokyo as the report in nature.

The papers shown above are diffrent from the 12 paperes, and the first authers are other persons. The work of the CELL paper is done in a famous US lab, by one of graduates of Dr Taira's lab. The EMBO paper was witten by another person.

We found further suspious papers of the groupe.

[TairaKawasaki]

Please leave your comment


FIG. 4.
Cells in wells and Western blotting analysis of cells after transfection with specific siRNA expression vectors. The target of each vector is indicated. The experiments were performed under the same conditions as those shown in Fig. 2. N1, negative control 1, siRNA expression vector targeted against Renilla luciferase; N2, negative control 2, siRNA expression vector targeted against HCV. pU6i indicates the empty siRNA expression vector.

[TairaKawasaki]

Hi guys!


i need responce.


thanks

[TairaKawasaki]

they had already been fired, but still she alive in aist in japan.