Login

Join for Free!
114033 members


dsRNA effect on adult neural stem cells

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

dsRNA effect on adult neural stem cells

Postby TairaKawasaki » Thu Apr 20, 2006 5:33 pm

How do you think dsRNA effect on adult neuron

We have found a strange coincide in Fig.4 on
Cell. 2004 Mar 19;116(6):779-93.
MID:15035981

Please check it by your own eyes.
Attachments
up4774.jpg
(200.19 KiB) Downloaded 481 times
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

Postby LilKim » Thu Apr 20, 2006 8:54 pm

hmm... i don't know the conditions or background for this figure

( and I don't have time to pub med it... at the moment.)

Do you know if this is a RNAi response?
User avatar
LilKim
Coral
Coral
 
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm

Postby cartener » Fri Apr 21, 2006 10:23 am

haha, good!
I have a question, who can answer me:
what is the definition of noncoding RNA? is that one do not have the genetic code ATG or this RNA display it.s function at the RNA lever?
cartener
Garter
Garter
 
Posts: 2
Joined: Fri Apr 21, 2006 9:50 am


Another one from the same Lab

Postby TairaKawasaki » Fri Apr 21, 2006 1:41 pm

An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease
Attachments
699860.jpg
(B) Expression of PKR mRNA in cells transfected with the empty vector (mock) or the Rz 49, Rz 47 or Rz 68 expression vector. PKR mRNA was detected by RT?PCR with primers specific for PKR (upper). GAPDH mRNA served as an internal control (middle). Expressi
(137.64 KiB) Downloaded 141 times
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

Postby TairaKawasaki » Fri Apr 21, 2006 2:48 pm

In 2005, among many other cases, a prominent Japanese scientist who published two major papers in Nature (H. Kawasaki and K. Taira Nature 423, 838?842; 2003 and Nature 431, 211?217; 2004) could not produce the corresponding experimental data. The first paper had already been retracted (Nature 426, 100; 2003) and the second corrected (Nature 431, 878; 2004). An investigation panel asked Taira to submit samples and notebooks relating to the experiments, but the researcher in his lab who ran the experiments did not have them. It is obvious that references to those papers will appear, be it only to state that they are likely to be faked, but this will obviously increase the Impact Factor of the journal... In the same way we can see in october 2005: Retraction: RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription
Vera Schramke, Daniel M. Sheedy, Ahmet M. Denli, Carolina Bonila, Karl Ekwall, Gregory J. Hannon and Robin C. Allshire
Nature 435, 1275–1279 (2005)

In spring 2006, investigation about RNA work in Taira's group (at least 12 papers published in quite "visible" journals are probably fakes).
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

Postby LilKim » Fri Apr 21, 2006 3:24 pm

hmm??? where did yo hear that? Is it on the nature website? I'm really curious because I read that paper recently. "induction of DNA menthlyation and gene silencing by short interfering RNA's in human cells" by kawasaki and taira
User avatar
LilKim
Coral
Coral
 
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm

Postby LilKim » Fri Apr 21, 2006 8:36 pm

never mind.. I found the reference thanks!
User avatar
LilKim
Coral
Coral
 
Posts: 436
Joined: Mon Mar 20, 2006 8:36 pm

Postby TairaKawasaki » Fri Apr 21, 2006 11:29 pm

All the 12 papers indicated in natutre are written by Dr. Kawasaki, an assistant researcher working for Dr. Taira at Univ of Tokyo as the report in nature.

The papers shown above are diffrent from the 12 paperes, and the first authers are other persons. The work of the CELL paper is done in a famous US lab, by one of graduates of Dr Taira's lab. The EMBO paper was witten by another person.

We found further suspious papers of the groupe.
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

J. Biol. Chem., Vol. 280, 25687-25696, 2005

Postby TairaKawasaki » Thu Apr 27, 2006 11:26 pm

Please leave your comment


FIG. 4.
Cells in wells and Western blotting analysis of cells after transfection with specific siRNA expression vectors. The target of each vector is indicated. The experiments were performed under the same conditions as those shown in Fig. 2. N1, negative control 1, siRNA expression vector targeted against Renilla luciferase; N2, negative control 2, siRNA expression vector targeted against HCV. pU6i indicates the empty siRNA expression vector.
Attachments
FIG. 4..jpg
(243.08 KiB) Downloaded 251 times
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

?

Postby TairaKawasaki » Mon Jan 15, 2007 1:24 pm

Hi guys!


i need responce.


thanks
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm

Re: dsRNA effect on adult neural stem cells

Postby TairaKawasaki » Sun Sep 19, 2010 1:09 pm

they had already been fired, but still she alive in aist in japan.
TairaKawasaki
Garter
Garter
 
Posts: 8
Joined: Wed Apr 19, 2006 7:15 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 4 guests

cron