Debate and discussion of any biological questions not pertaining to a particular topic.
no, Karry Mullis used E.Coli polymerase for his PCR experiments. It was Saiki later in 1988 who used thermostable poymerases here and made PCR a revolution
Ok, you might get a softer landing from other people on this one, but this is one of my pet peeves. Not so much that you corrected me(happened before, will happen again), but that you didn't provide any proof as to the validity of your statement. Since this is a website dedicated to science, wouldn't it be prudent to provide some links or book references to back up your contradiction of my statement? Don't worry, I'll do the work..this time.
Pay particular attention to the Idea Number Two: Thermostable Taq Polymerase heading near the bottom of the page
Now, I did google searches in multiple parameters and Saiki is listed as the primary author on the seminal 1988 article Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase., K. Mullis is also listed as an author. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=2448875
Seems there is some controversy in this area, but since the reference to Mullis as a the discoverer is on wikipedia and the pubmed article lists Saiki as the primary author, I would lean more towards Saiki. Now, the article that I read when I was doing my (cursory) initial look listed Mullis as the discoverer, hence why I listed it.
Now, the point of my little rant here isn't to start a fight over the Taq issue, but to point out that this is a website dedicated to biology, not slashdot or some other mass market forum. We're supposed to be biologists of some form or another, whether students, professionals, or profs and I think its fair to expect a higher level of discussion than simple contridictory statement.
That is all...for now.
'It is futile to pretend to the public that we understand how an amoeba evolved into a man, when we cannot tell our students how a human egg produces a skin cell or a brain cell!'
Dr Jérôme J. Lejeune
Jelanen, I'm glad to see your last post (I liked it). It shows the personality of a true Biologist, he he. All the people who want to dedicate their lives to Science have to be nonconformists. This is one of the basis of research.
You shouldn't base a fact on one simple statement. You have to get a lot of good information (and read it, of course), to research, to pry, to link causes and to make hypothetical cases.
Nice post . See you!
If you felt offended with my statement. I was just making my point what I have read about the discovery. I mostly refer Sambrook et al for molecular biology topics and I got the point from it. And I agree science topics are not for fight but for discussion. And I am very inexperienced as you all must be in this field , so if you disliked something then I am sorry.
As for Karry Mullis, he is indeed credited as the discoverer, but my point is can we neglect the ideas og H.G. Khorana and the use of thermostable polymerase in PCR, which made it so successful.
I just want to know your views, hope you'll answer
If we can include fictional biologists I would have to say Kaye Lang from Darwin's Radio by Greg Bear.
Otherwise, I rather like Craig Venter. Possibly just because few other people do. By playing the competition and trying to run an independent, profit-based genome sequencing operation, he helped to speed up the mainstream HGP.
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