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Papanicolaou staining

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Papanicolaou staining

Postby pdavis68 » Mon Apr 03, 2006 8:50 pm

I'm a bit confused about hot to do pap stains. I've got Haematoxylin.

My understanding is that I also need OG-6 and Eosin Azure.

My understanding is that:

OG-6 is a mixture of Orange G and Phosphotungstic Acid

Eosin Azure is a mixture of Eosin Y, either Light Green SF Yellowish or Fast Green FCF, and Bismark Brown Y.

All I have at the moment is Haematoxylin and Eosin Y. So my question is, am I correct that these are the things that I need and if so, where can I find them in relatively small amounts. I can get stuff from Fischer Scientific in 1L bottles, but that's WAY more than I need.

Are there ready made mixes that I can get? I heard there are variations of the Pap stain. Are there cheaper variations? I know haematoxylin wasn't cheap.

Thanks.

Pete
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