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Enzyme help!!!

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Enzyme help!!!

Postby mr_sheldo » Mon Mar 21, 2005 4:57 am

I am a college student and I am needing help with some research. I am trying to find an enzyme that can remain active in relatively acidic environments, as well as basic environments. I know that usually when you plot enzyme activity with ph you get a very steap curve, the high point being that of the optimum enzyme activity. I am looking to find an enzyme that has a broader "curve" that will remain active in both environments without that sudden decrease or failure to perform just outside of the optimum pH.
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Postby Poison » Mon Mar 21, 2005 4:40 pm

I haven't heard about an enzyme like that.
Has anyone?
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Postby zerotimbo2001 » Wed Mar 23, 2005 8:28 am

Hi there

In my research (PhD) I have isolated an enzyme called Actyl-0-transferase thatde-acetylates various toxins as a self-protection mechanism. That is to sayby reducing this toxin (Deoxynivalenol) is protects from "self harm". This enzyme is up-regualted in environments with low pH, but maintains its ability in higher pH regions if indeed at a lower concentration... its quite unique in this ability and I'm not sure it conforms to your requirements but it may help

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Postby Inuyasha » Thu Mar 24, 2005 8:09 pm

Ah enzymes. There are quite a few, I think. Most come from archeas. I remember my teacher saying something on that. Just check for some archeas and see if any of their enzymes fit. Or yeah, One of the enzymes you are looking for is used for DNA recombination.
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Postby biostudent84 » Thu Mar 24, 2005 8:47 pm

Inuyasha wrote:Ah enzymes. There are quite a few, I think.


Uh, Inuyasha? Every unique chemical reaction performed in any organism requires its own unique enzyme.
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Postby MrMistery » Fri Mar 25, 2005 8:29 pm

@Inuyasha
What enzime? The replication of Dna requires a lot of enzimes. I have abot 15 of them in a book called "Basic genetics" so you might try being more specific if possible
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