Transfection

[chancer]

# Should I always split my cells at their mid log phase? Or does it only matter at transfection? How do I know that my cells are in the mid log phase or not too long in the plateau?

# Where should I prepare the media? In the laminar flow hood or on an opened table (risks for contamination?) ?

#If medium with serum contains trypsin inhibitor. What should I use to dislodge my cells?

#Should I always have HCO3- in the medium and what for?

#How do I know the minimum of cells that give a quick entry into log phase growth?

Thanks.