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gel extraction

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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gel extraction

Postby Shirley » Sun Feb 05, 2006 8:02 am

Hi,
The weight of my gel slice weighs 428 mg and the maximum weight per spin column is 400mg. What is the consequence of loading everything into one spin column? :roll:
Shirley
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Postby pjansen » Mon Feb 06, 2006 2:43 pm

you can do two things:
1. put everything over 1 column (this is what i should probably do)
2. cut the slice in two peaches of 214 mg and use two column

to be honest, i never weight slices, if you were using the Qiagen kit, just add 450 ul buffer QC and wait till the slice has disolved

grtz
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