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Nucleoside Triphosphate Binding to complimentary Nucleotide

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Nucleoside Triphosphate Binding to complimentary Nucleotide

Postby poncecernesto » Tue Apr 11, 2017 6:51 pm

I've been wondering what process the nucleoside triphosphate binds to the complimentary nucleotide. My book just says polymerization, but it shows two of the phosphates leaving, and it binding to the 3' end's OH. Is it ATP? Because I don't see water involved, or is it just natural interactions?

Also, if anyone has extra time, my teacher asked me what problems can be faced with replicating bacterial chromosomes, but I'm not sure why there's any difference. I know that bacteria can replicate using circular DNA, but is the problem that they might not be able to un-attach the replicated strands, so it leads to supercoiling? Is there anything else that could go wrong?

Thanks!
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Re: Nucleoside Triphosphate Binding to complimentary Nucleot

Postby claudepa » Tue Apr 18, 2017 9:35 am

If you are speaking of DNA synthesis, binding between complementary nucléotides (Watson Crick rules) is done through hydrogen bonding, 2 bonds for AT and 3 bonds for GC. Polymerisation takes place within the new strand and is not related to the complementary bases of the old strand. For bacterial DNA replication I see indeed no particular problem, except that, as you say it is a circular DNA (human chromosomes are linear DNA) and then replication has to stop when the circle is finished. If it does not stop it will create by replication many new circles. This problem does not exist with linear DNA.
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