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passage number in mammalian cells

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passage number in mammalian cells

Postby forumer » Tue Mar 15, 2005 9:10 pm

if a cell line has a maximum of 10 passages before it changes character and i only have done 4 passages of it and made the frozen stock of some of the cells. how many passages does this frozen stock have if i thaw it and passage it? will it be only 6 passages left or 10 passages? :roll:

thanks for any inputs.
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Postby DevGrp » Wed Mar 16, 2005 10:48 am

It all depends on the cells and the characteristic you are studying.
Some cells (Hek293 ) can be passaged many many times, others can't. I tend to make a lot of frozen aliqouts of a new cell line as soon as we get it and then go back to that P1 whenever I suspect the cells are getting a bit old. For Eahy296 cells for instance by passage 20 their expression of estrogen receptor beta (which we were studying) had droped 1000 fold. As I said other cells can go on much longer.
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Postby forumer » Wed Mar 16, 2005 12:30 pm

thanks. :)
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Postby lpojoga » Tue Feb 20, 2007 8:00 pm

DevGrp wrote:It all depends on the cells and the characteristic you are studying.
Some cells (Hek293 ) can be passaged many many times, others can't. I tend to make a lot of frozen aliqouts of a new cell line as soon as we get it and then go back to that P1 whenever I suspect the cells are getting a bit old. For Eahy296 cells for instance by passage 20 their expression of estrogen receptor beta (which we were studying) had droped 1000 fold. As I said other cells can go on much longer.


Hi there - I noticed you mentioned that Eahy296 cells massively drop their ERbeta number with passage. Did you have that data published anywhere? if not, did you guys have a decay curve that you would care to share? I too am using these cells and am looking at a nuclear receptor, and would like to know more about the decay curve (how steep the decay was, what passage would be a mid-point from this point of view, etc). Please let me know. Thank you so much!
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Postby Vagabond » Wed Feb 21, 2007 2:21 pm

All cell lines are modified in some way that allows them to grow and grow. 10 passages seems a little low to me for cell lines now for primary cell cultures I would think that, that number seems about right. And remember all cell lines are just that cell lines and have all been altered in some way so it is good scientific practice to check your cells to confirm that the pathway or protein you are studying is functioning properly.

And to answer your question – I suggest that you consider the thawed cells to be passage number 5.


Good luck
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Postby Priscilla » Fri Feb 23, 2007 12:51 am

It would be 5 passage left.
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