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Cell Culture Contamination Air particle quantity/Size?

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Cell Culture Contamination Air particle quantity/Size?

Postby NathanielZhu » Sun Oct 25, 2015 8:21 pm

I'm building a simple DIY hood using a 20x20 box fan, an air filter (going to test multiple filters to find the minimum quality of filter and get the job done), and an aluminum box. 
I'm a bit unsure of how good the BSL2 biosafety cabinets are at filtering contaminants. Clearly, my cultures don't get contaminated in a BSL2 biosafety cabinet but of course, I can't imagine that the cabinet filter's out 100% of contaminents. This guy has a air particle detector. https://www.youtube.com/watch?v=kH5APw_SLUU. The best filter he has leaves 400 particles in the air. I assume a BSL2Cabinet also leaves about the same. 

So,
Does it take a single particle to contaminate a Culture?

If not, what are the sort of ranges that would be safe for cell culturing?

Then, what sort of MERV rating should I go for? I'm leaning towards merv 13. It gets much more expensive from there. 
This site seems to claim that they can filter out all bacteria
https://filterbuy.com/pla/product/20x20 ... Al138P8HAQ

Cell Culture Contamination Air particle quantity/Size? - ResearchGate. Available from: https://www.researchgate.net/post/Cell_ ... ntity_Size [accessed Oct 25, 2015].
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