Biology-Online • View topic - Total protein concentration before Elisa

Join for Free!
122498 members

Total protein concentration before Elisa

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Total protein concentration before Elisa

Postby Steven2015 » Wed May 13, 2015 2:18 am

I want to measure a protein in Bronchoalveolara Lavage Fluid (BALf) which is the lining fluid from the bronchial and alveolar epithelium. It is aspirated with saline through Bronchoscope. I am going to do elisa, which i get from here ( ... pment.html), but i don't know what is the point of measuring the total protein concentration before Elisa. The most papers i have reviewed, using Elisa in BALf, have results in pg/ml or ng/ml or ug/ml and they have not performed an assay for the total protein concentration.
Posts: 19
Joined: Mon Feb 09, 2015 8:38 am
Location: Shirley, NY 11967, USA

Postby venus666 » Thu Oct 15, 2015 7:30 am

Generally , the test information you want can be read in the instruction book of ELISA, good luck.
Posts: 8
Joined: Wed Jun 24, 2015 9:36 am

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest