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## DNA Conc

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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### DNA Conc

How do you work out DNA concentration?

I have spec DNA- result 0.023
Dilution 1/1000

How do i translate this to ng/ul?

Thanks
angela.morris
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Joined: Thu Mar 10, 2005 5:31 pm

Do you have volume or anything else?

Garter

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Joined: Thu Mar 10, 2005 10:12 pm
Location: Kerman, California

Hi angela.morris

You are right your concentration is 1150ug/ml

you have to multiply this number for a coeffiicient that is 50 for DNA and for your diluition factor (1000 in your case)
0.023 x 50 x 1000= 1150ug/ml

(This formula is known as Lambert-Beer)

To convert into ng/ul is very simple: is exactly the same 1150ng/ul

Ciao
Dott. Berrino ^--^

Dott. Berrino
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Location: BioMol Room (Italy)

Sounds correct. Just to add that the conversion to ng/ul from ug/ml is exactly the same because ng is 10-9, ug 10-6 and ml 10-3. Thats 10 to the power of -9 etc. Therefore 10-6/10-3 is same ratio as 10-9/10-6. Works if you do the maths.
Please correct me if im wrong.

Chris4
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Location: UK

Perfect Chris4!!!
Dott. Berrino ^--^

Dott. Berrino
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Location: BioMol Room (Italy)

### researchers

Oh I didn't know that formula, how many of us here really happen to use them? ? ?

I think 2 out of 5 , right ? ? ?

hrushikesh
2810712
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I used it. In my 1st and 2nd year of university. Everyone who was doing a biology related degree at the uni had to use it for the basic genetics and molecular biology practicals.

Chris4
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Joined: Mon Mar 07, 2005 4:56 pm
Location: UK

### what

What ? I thought u are preuniv. I'm preuniversity student.
sorry this is not off topic forum,but Iwas just curious, sorry.

hrushikesh
2810712
King Cobra

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Joined: Thu Jan 20, 2005 12:19 pm

### Re: what

2810713 wrote:What ? I thought u are preuniv. I'm preuniversity student.
sorry this is not off topic forum,but Iwas just curious, sorry.

hrushikesh

Im new here. I've never mentioned anything about university before.
Im in my 3rd year of a 4 year Cell Biology degree. So ive only got just over a year left

Chris4
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Posts: 286
Joined: Mon Mar 07, 2005 4:56 pm
Location: UK

best luck!!! cellbio is great ! ! !

hrushikesh
2810712
King Cobra

Posts: 697
Joined: Thu Jan 20, 2005 12:19 pm