Login

Join for Free!
119198 members


use of a bacterial protease inhibitor cocktail with mammalia

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

use of a bacterial protease inhibitor cocktail with mammalia

Postby rpwdoc » Tue Aug 26, 2014 10:08 pm

Dear fellow biochemists,

I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical representative recommended my using "539134 | Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem" as a good general protease inhibitor cocktail.

I have another protease inhibitor cocktail which includes: AEBSF, bestatin, E-64, and pepstatin A (E.coli protease inhibitor cocktail) but it does not include the aprotinin or the leupeptin inhibitors. Is there a risk of proteolysis in a mammalian cell lysate without these latter 2 inhibitors? The specific cell types which we are using are: HUVEC (human umbilical vein endothelial cells) and HT-29 adenocarcinoma cell line.

Dr Robert Weinberg
Dept of Biology, MIT
email: rweinber@mit.edu
fax: 617-977-0902
rpwdoc
Garter
Garter
 
Posts: 1
Joined: Tue Aug 26, 2014 10:05 pm

Postby JackBean » Sat Nov 08, 2014 5:18 pm

well, it would be advisable to add them
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5690
Joined: Mon Sep 14, 2009 7:12 pm


Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 2 guests