Login

Join for Free!
118293 members


Gene knockdowns in the wasp Nasonia vitripennis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Gene knockdowns in the wasp Nasonia vitripennis

Postby jonmoulton » Fri Mar 07, 2014 5:57 pm

This paper describes gene knockdowns in the wasp Nasonia vitripennis by Morpholino antisense oligos, delivered by microinjection.

Dual mode of embryonic development is highlighted by expression and function of Nasonia pair-rule genes. Rosenberg MI, Brent AE, Payre F, Desplan C. eLife. 2014;3:e01440 doi:10.7554/eLife.01440

"Although parental RNAi in Nasonia is effective for maternal and early zygotic genes ..., it often does not provide significant knockdown of later-acting genes. To overcome this limitation, we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon-intron junction in the homeobox. These two independent morpholinos are expected to disrupt Nv eve activity and indeed result in comparable phenotypes."

http://elife.elifesciences.org/content/3/e01440
User avatar
jonmoulton
Viper
Viper
 
Posts: 431
Joined: Fri Feb 15, 2008 5:38 pm
Location: Philomath, Oregon, USA

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests