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Slicing frozen chromosomes with lasers and scanning with SEM

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Slicing frozen chromosomes with lasers and scanning with SEM

Postby enarees » Sat Feb 22, 2014 5:37 am

Idea for ​​whole genome sequencing is presented in the title.


It is said so that WOMEN understand not only illuminated Freemasons.
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Postby JackBean » Mon Feb 24, 2014 10:20 am

What exactly do you imagine by "slicing frozen chromosomes"?

Do you know, what is the resolution of SEM? Do you know the size of nucleobasis?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby enarees » Tue Feb 25, 2014 6:05 pm

JackBean wrote:What exactly do you imagine by "slicing frozen chromosomes"?

Do you know, what is the resolution of SEM? Do you know the size of nucleobasis?



0.5 nm is achievable.
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Postby JackBean » Wed Feb 26, 2014 9:33 am

usually 1 nm and higher. Size of atoms (C, N) is 70 pm and smaller. That's 20-times less.

However, you may be interested in the new one-molecule sequencing methods.

And separation of chromosomes by dissection by laser is also nothing new.
http://www.biolib.cz/en/main/

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Re:

Postby enarees » Wed Feb 26, 2014 6:57 pm

JackBean wrote:usually 1 nm and higher. Size of atoms (C, N) is 70 pm and smaller. That's 20-times less.

However, you may be interested in the new one-molecule sequencing methods.

And separation of chromosomes by dissection by laser is also nothing new.



This is not a problem again.

After scanning slices should not be necessary.
http://ieeexplore.ieee.org/xpl/login.jsp?tp=&arnumber=5292236&url=http%3A%2F%2Fieeexplore.ieee.org%2Fiel5%2F5238710%2F5292053%2F05292236.pdf%3Farnumber%3D5292236


If you want to make an artificial chromosome, it must build in this way to keep isomerism and quantum condition!
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