help with phospholipase A

[NDarinzo]

Hey guys, for my AP Biology year-long project I'm doing an experiment on how aspirin effects prostaglandin levels in a particular area by inhibiting COX-2. So I'm trying to figure out the rates of prostaglandin production and how they decrease as the Acetylsalicylic acid is put into the cell culture. Basically I have to figure out how I can get the tissue to produce prostaglandins (they have a very short half-life, so they're only produced when needed). I've traced the path of prostaglandin production up to phospholipase A2, but I can't find anything that has to do with it, I need to know what causes it to start catalyzing reactions that end up producing the prostaglandins. Anyone knowledgeable in this area?

Another thing I need to do is determine the enzyme concentrations, and I have access to a lab so I'm sure they know what to do, however right now I'm required to write a procedure for my bio teacher. I've looked all over the internet and I can't figure out how you can determine enzyme concentration! It would be great if someone could help me out, by posting here or emailing me at NDarinzo@gmail.com.

Thanks, Nicholas

[Enzyme]

Hello Nicholas. How is going your project? I can send you some bibliographic references about prostaglandines and phospholypase A if you want, but I can't do more, sorry .

I recommend you to search in MEDLINE database (or in PUBMED).

About determining the enzyme concentrations, I can help you a bit (I have to take a look to my notebooks again, he he). I have some notes written (but in Spanish) that I can send you if you want them (my e-mail address is dj-enzyme@hotmail.com).

Good luck in your project and keep me informed about it.

See ya!