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A dilution question about RNA

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A dilution question about RNA

Postby golgicik » Mon Feb 03, 2014 6:30 pm

Hello,

I have a basic and probably silly question. I am isolating RNA with TRIzol. I have performed the basic steps and resolve the pellet in 50 uL water. I ve measured the concentration and it was 3313 ng/uL (Around 165 ug). Now my next step is DNase treatment. It says 1 unit of enzyme for 1 ug of RNA in 10 ul reaction mix. I would like to dilute my RNA to 100 ug and use 100 unite enzyme in 1000 uL reaction mix. Is this logic true and if it is how I am going to do the dilution?

Thanks!
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Re: A dilution question about RNA

Postby JackBean » Thu Feb 06, 2014 4:31 pm

Why, for God's sake would you make a DNase treatment in 1ml??? :shock:

You would take around 30 ul of your RNA (100ug / 3.3ug/ul), add the DNase worth few tens of bucks at least, 100 ul of 10x buffer and water up to 1 ml.
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