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URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

Postby microbiologyhelp » Wed Nov 20, 2013 6:09 pm

Hey everyone, was just requiring a bit of help for my microbiology class,recently have done biochemical tests to help identify this unknow organism, would be very appreciative if anybody can help me identify it. thanks so much


GRAM NEGATIVE- clustered in chains, splotches, and red/pink. Blue Vegetative cells.
SPORE REACTION (-)
ACID FAST STAINING (-)
NUTRIENT BROTH CULTURE- Quite turbid, slightly cloudy, no skin
NUTRIENT AGAR STREAK- Shape – Cocci, Yellow, 0.2-0.5m Glossy
NUTRIENT AGAR STAB: Aerobic
GELATIN STAB: No Proteolysis
THIOGLYCOLLATE MEDIUM- Anaerobic
SEMI SOLID (Sloppy) Agar – Anaerobic
BLOOD AGAR PLATE: B-Haemolysis
HUGH AND LEIFSON’S O/F TEST: Negative
ANTIBIOTIC SENSITIVITY- Resistant.
CATALASE- NEGATIVE
OXIDASE- POSITIVE
FERMENTATION:
OF GLUCOSE – NEGATIVE, MANNITOL – NEGATIVE, DULCITOL – NEGATIVE
(A) ACID PRODUCTION MEDIUM YELLOW BUT NO GAS IN DURHAM TUBE – SUCROSE, LACTOSE, MALTOSE.
STARCH HYDROLYSIS – NEGATIVE
CITRATE UTLISISATION – NEGATIVE
INDOLE PRODUCTION – NEGATIVE
HYDROGEN SULPHIDE PRODUCTION – NEGATIVE
METHYL RED TEST – NEGATIIVE
VOUGES PROSKAUER TEST – NEGATIVE
UREA HYDROLYSIS – NEGATIVE


THANK YOU
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Re: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

Postby jonmoulton » Fri Nov 22, 2013 4:49 pm

Looks like a job for Bergey's Manual of Determinative Bacteriology.
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Re: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

Postby microbiologyhelp » Wed Nov 27, 2013 5:31 pm

jonmoulton wrote:Looks like a job for Bergey's Manual of Determinative Bacteriology.


hey cheers for the reply, can you please help me with identifying it?:)
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Postby Cat » Fri Nov 29, 2013 5:17 pm

"GRAM NEGATIVE- clustered in chains, splotches, and red/pink. Blue Vegetative cells."

- Rods or Cocci???

"THIOGLYCOLLATE MEDIUM- Anaerobic"

-Which anaerobe? (http://en.wikipedia.org/wiki/Thioglycollate_broth)

"SEMI SOLID (Sloppy) Agar – Anaerobic"

- This media is usually used to test motility.

"ANTIBIOTIC SENSITIVITY- Resistant."

- To what???

"HUGH AND LEIFSON’S O/F TEST: Negative"

- It cannot be negative. It's either Oxidative, Fermentative, or both.

"METHYL RED TEST – NEGATIIVE"

- Did you re-test after additional 48HRS of incubation?
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Re:

Postby microbiologyhelp » Sun Dec 01, 2013 11:36 am

Cat wrote:"GRAM NEGATIVE- clustered in chains, splotches, and red/pink. Blue Vegetative cells."

- Rods or Cocci???

"THIOGLYCOLLATE MEDIUM- Anaerobic"

-Which anaerobe? (http://en.wikipedia.org/wiki/Thioglycollate_broth)

"SEMI SOLID (Sloppy) Agar – Anaerobic"

- This media is usually used to test motility.

"ANTIBIOTIC SENSITIVITY- Resistant."

- To what???

"HUGH AND LEIFSON’S O/F TEST: Negative"

- It cannot be negative. It's either Oxidative, Fermentative, or both.

"METHYL RED TEST – NEGATIIVE"

- Did you re-test after additional 48HRS of incubation?




Hey thanks for the reply I did further research and found out that...
Gram reaction:+
Shape: cocci
Acid-fast: -
Spores: -
Motility: -
Grows in air: +
Grows anaerobically: +
Catalase: -
Oxidase: -
Acid from glucose: +
OF test: : F
Gas from glucose: -
Acid from sucrose: +
Acid from lactose: +
Acid from maltose: +
Acid from mannitol: -
Acid from dulcitol: -
Nitrate reduction: +
Citrate utilisation: -
Indole production: -
H2S production: -
Methyl red test: -
Voges Proskauer test: -
Urea hydrolysis: -
Gelatin hydrolysis: -
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Postby Cat » Sun Dec 01, 2013 8:02 pm

You have a problem. Most of the tests you preformed were designed to ID gram negative bacteria. In your earlier post you said that you got beta hemolysis, so you can say that it's "beta hemolytic streptococci" or you can do additional tests to determine which one. See flow charts:

http://loudoun.nvcc.edu/vetonline/vet13 ... cocci2.gif
http://img.docstoccdn.com/thumb/orig/9217067.png
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Re:

Postby microbiologyhelp » Mon Dec 02, 2013 9:12 pm

Cat wrote:You have a problem. Most of the tests you preformed were designed to ID gram negative bacteria. In your earlier post you said that you got beta hemolysis, so you can say that it's "beta hemolytic streptococci" or you can do additional tests to determine which one. See flow charts:

http://loudoun.nvcc.edu/vetonline/vet13 ... cocci2.gif
http://img.docstoccdn.com/thumb/orig/9217067.png


Ignore my previous comment about gram negative, just realised looking back at my lab reports that its gram positiive, almost certain it is beta heamolysis really struggling to find this bacterium out :/
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Re: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

Postby sosippus » Wed Sep 24, 2014 7:49 pm

jonmoulton wrote:Looks like a job for Bergey's Manual of Determinative Bacteriology.


This!
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