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Using oxidizing agents in gram stain

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Using oxidizing agents in gram stain

Postby ccBiologist » Sun Sep 22, 2013 2:44 pm

Considering the fact that Iodine is an oxidizing agent, what would happen if I replaced iodine with another oxidizing agent during the gram staining process ? So far I know that the purpose of the iodine is to form a complex with the crystal violet in order to enable the cell wall of the bacteria to retain the dye but certainly there must be other oxidizing agents that can replace iodine ?
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Postby Cat » Sun Sep 22, 2013 3:13 pm

Interesting, logical, and probably possible. However, what is the point? Iodine is very cheap and non-hazardous, so, why would you want to change it?
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