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Cell culture

Discussion of all aspects of cellular structure, physiology and communication.

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Cell culture

Postby Nadaezz » Sat Sep 21, 2013 1:59 pm

I have a question and I need your help badly,
I am having a cell culture without a cell line.. only the samples to be tested with DMEM suplemented with 10% FBS.. It has to be incubated in 5%CO2 and 95% air.. but there is no CO2 incubator available.. So how can I provide these conditions to my culture knowing that I am culturing in 25-ml tissue culture flasks with filter cap?
I need the reply urgently
Nadaezz
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Postby Cat » Sun Sep 22, 2013 3:19 pm

Check with other labs. One of them should have the incubator.
Cat
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