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indole test reaction

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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indole test reaction

Postby magandangbutiki » Fri Aug 02, 2013 7:53 am

guys, i used Lauryl Tryptose Broth for indole test, since the Techical Data Sheet of LTB says it can be used for such biochemical test ( it has Typtose), i incubated the 5 tubes (with inoculum) at 44.5 degrees and after 24 hrs. i dropped kovacs reagent to the tubes, after a while 1 tube has a red ring while the other tubes has green ring. And then after a while the tube with the red ring suddenly turned greenish..can anybody tell me what happened? can i report the 1 tube with red ring as positive for e.coli?
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