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The role of DNA polymerase

Genetics as it applies to evolution, molecular biology, and medical aspects.

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The role of DNA polymerase

Postby Dunceinthecorner » Tue Nov 15, 2005 9:00 pm

Im hoping someone can help me with this question.
What role does DNA polymerase play in the process of plasmid transfer by conjugation?
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Postby Geordie Boy » Wed Nov 16, 2005 1:09 pm

I'm afraid i dont know anything about plasmid transfer. However i am pretty sure that DNA Polymerase is an enzyme which can reconstruct DNA from RNA or from small amounts of DNA.

I am not 100% on this but if there is anything in the process that involves any of the abouve then that will be it.
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role of dna polymerase

Postby lara » Thu Nov 17, 2005 8:11 am

1,An F+ plasmid inserts into the donor bacterium's nucleoid to form an Hfr male.
2.The sex pilus adheres to an F- female (recipient). One donor DNA strand breaks in the middle of the inserted F+ plasmid.

3.The sex pilus retracts and a bridge forms between the two bacteria. One donor DNA strand begins to enter the recipient bacterium. The two cells break apart easily so the only a portion of the donor's DNA strand is usually transferred to the recipient bacterium.

4.The donor bacterium makes a complementary copy of the remaining DNA strand and remains an Hfr male. The recipient bacterium makes a complementary strand of the transferred donor DNA.
i guess it's here where dna polymerase comes into play during plasmid transfer.
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