Biology-Online • View topic - calcium measurement- help!!

Join for Free!
122511 members

calcium measurement- help!!

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderator: BioTeam

calcium measurement- help!!

Postby reulita10 » Tue Jul 16, 2013 12:34 pm

Hello everyone,
i'm looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght).
I understand the equation with Kd, Fmin, Fmax etc. but don't know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it?
thanks in advance!
Posts: 2
Joined: Tue Jul 16, 2013 12:24 pm

Return to General Discussion

Who is online

Users browsing this forum: No registered users and 1 guest