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BL21 colony as a template

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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BL21 colony as a template

Postby sureshG » Mon Jul 15, 2013 10:14 am

Hi..,

I am working on E Coli metabolites. I want to amplify a 2kb product from BL21 cells. I do not have genomic DNA of BL21 so, I want to do colony PCR where colony as a template. Can anyone suggest me whether it is possible to amplify the product from colony PCR where colony as a template.
sureshG
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