Biology-Online • View topic - HIS3-MX6 selective marker in S.cerevisiae

Join for Free!
122503 members

HIS3-MX6 selective marker in S.cerevisiae

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

HIS3-MX6 selective marker in S.cerevisiae

Postby cathbr » Fri Jul 05, 2013 6:51 pm


I am facing some problems when I use HIS3-MX6 selection for budding yeast transformations. I was wondering if anybody had similar problems and would have a solution (like a specific way to transform yeasts with plasmids containing HIS3-MX).
I try to transform an integrating PCR fragment that contains HIS3-MX6 marker but I don't recover any transformants on -HIS drop-out plates. (I do not have any problems for my other transformations).

thanks for your help.
Posts: 7
Joined: Mon Mar 12, 2012 1:38 pm

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest