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Fixing CFSE labeled cells

Discussion of all aspects of cellular structure, physiology and communication.

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Fixing CFSE labeled cells

Postby BiolMC » Tue Jun 11, 2013 12:34 pm

Hi all,

I want to label cells with CFSE, culture them, collect cells at different time points and analyse them all at once by flow cytometry. I was planning to fix the cells with 75% ethanol after collecting them. Is this possible / reliable? Or does fixation / storage affect the signal?
And another thing, how sensitive is the CFSE signal to light? Do I have to wrap my 6-wells plate in aluminium foil?

greets,

Menno
BiolMC
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