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apolipoprotein in HDL

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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apolipoprotein in HDL

Postby staysweet » Wed May 15, 2013 11:56 am

Hi all

I'm not a bio major so I don't know about proteins and lipids as much as you guys do.

My question is about apolipoproteins in HDL.

I know that apoA1 is the most abundant apolipoprotein species in HDL but is there apoA1 in LDL or VLDL as well?
If there is, do they share the same exact structures from that of HDL?

If I were to use antibodies to separate apoliproteins 1A of HDL from plasma, would I have to separate HDL out of plasma first or can I juss directly use the antibodies in plasma?

Thank you
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Postby JackBean » Thu May 16, 2013 3:11 pm

Obviously not http://en.wikipedia.org/wiki/Low-densit ... #Structure

You could use e.g. centrifugation (although I don't know how stabile would the pellet be) to remove it from the plasma. But it should be possible to use directly the plasma.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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