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How can I add a kozak sequence in plasmid pMT/bip/V5-His A

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How can I add a kozak sequence in plasmid pMT/bip/V5-His A

Postby yiwi123 » Tue Mar 12, 2013 8:04 pm

Hi guys,

I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak sequence?

Thanks for your time.
Alex.
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Postby JackBean » Wed Mar 13, 2013 4:59 pm

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.
http://www.biolib.cz/en/main/

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