How can I add a kozak sequence in plasmid pMT/bip/V5-His A

[yiwi123]

Hi guys,

I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak sequence?

Thanks for your time.
Alex.

[JackBean]

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.