Join for Free!
121438 members

How can I add a kozak sequence in plasmid pMT/bip/V5-His A

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

How can I add a kozak sequence in plasmid pMT/bip/V5-His A

Postby yiwi123 » Tue Mar 12, 2013 8:04 pm

Hi guys,

I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak sequence?

Thanks for your time.
Posts: 1
Joined: Mon Jun 11, 2012 11:49 pm

Postby JackBean » Wed Mar 13, 2013 4:59 pm

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.

Cis or trans? That's what matters.
User avatar
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests