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Over expressing proteins in mammalian cells

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Over expressing proteins in mammalian cells

Postby simina » Tue Feb 19, 2013 9:24 pm

Can anyone tell me an advantage and a disadvantage of over expressing proteins in mammalian cells to discover their function or localisation?
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Postby JackBean » Wed Feb 20, 2013 1:32 pm

if it's mammalian protein than you have almost native conditions
However, it's not so easy to handle.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby nima12 » Mon May 06, 2013 3:08 pm

realy is very hard
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