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How a gene detect where to insert after injected to nucleus

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How a gene detect where to insert after injected to nucleus

Postby leminhduc93 » Sat Feb 09, 2013 9:30 am

Hello,

I mean how a gene after injected to a nucleus can detect where to insert itself.

Example : human have 23 chromosomes, i want to replace the gene ABC at the position XYZ of the 1st chromosomes with a new gene so it can production for new protein instead of old protein. After have a plasmid that included the targeting gene to transfer, how the gene to be inject can detect the position (which chromosome, which position) to injecting. Does it replace or just insert a new gene ? Or it do nothing with the original gene but just insert a gene like a new chromosome ?

I'm fairly new to this and i very stupid but i very like science, may be a stupid question but a big thank for some one can help me . Please help me.
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Postby JackBean » Sun Feb 17, 2013 10:48 pm

That depends whether you add some homology sequences and homology recombination will be used to incorporate that gene. Honestly, I don't know, what's the ratio of HR and NHEJ in human.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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