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Orientation of genes

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Orientation of genes

Postby Sue4 » Mon Jan 28, 2013 8:23 pm

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have placed in one direction and the marker gene has placed in the opposite direction. Is there any reason to do so?

Thank you very much :)
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Postby Sue4 » Mon Jan 28, 2013 9:03 pm

"to be cloned" part is not needed. Sorry

When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have placed in one direction and the marker gene has placed in the opposite direction. Is there any reason to do so?

Thank you very much
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Postby JackBean » Tue Jan 29, 2013 11:00 am

I'd say it's in opposite direction to abolish transcriptional fusion.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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