Biology-Online • View topic - Competent cell growth on all my plates (multi resistant)

Join for Free!
122501 members

Competent cell growth on all my plates (multi resistant)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Competent cell growth on all my plates (multi resistant)

Postby emadsadeghian » Fri Jan 04, 2013 12:27 pm

I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclon, Ampicilin an control. After overnight incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately.
Since I have kept my original stock of cells before making them competent, I did the same test on the original stack and i saw colonies only on the tetracyclin plates (XL1 Blue is resistant to Tet). This means the procedure of producing the competent cell has somehow made my Ecoli strand a multiresistant strain.
My question is if this is possible and if not why this has happened to my batch?
Posts: 2
Joined: Fri Jan 04, 2013 12:18 pm

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests