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The same old silly question about molarities

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The same old silly question about molarities

Postby bravebeaker » Wed Dec 26, 2012 3:35 am

Hi all,
I have a question and I would like you to help me figure it out.
Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this
20 mM MES
0.4 M mannitol
20 mM KCl
10 mM CaCl2
0.1% BSA
1.5% Cellulase and so on...
I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume to work with? lets say I need 10 ml of this enzyme solution with the mentioned contents.

Thank you very much for your help.
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Postby JackBean » Sun Dec 30, 2012 10:12 pm

just put everything into one beaker
http://www.biolib.cz/en/main/

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Re: The same old silly question about molarities

Postby bravebeaker » Wed Jan 09, 2013 7:22 am

Well Jack bean... I was waiting with anticipation for your reply actually but not kind of that reply.. very elaborative :P but thanks anyways :D
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Postby JackBean » Wed Jan 09, 2013 9:42 am

just weight all chemicals, put them into one beaker and let dissolve. That's how you make buffers.
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