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looking for a difficult gene

Genetics as it applies to evolution, molecular biology, and medical aspects.

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looking for a difficult gene

Postby iruka » Fri Dec 07, 2012 10:01 am

Hello all!!
I am trying to carachterize a gene that has been sequenced in related species, but there is no way to find it in my species. I have tried Genome Walker, RACE and even tried at the protein level with immunoprecipitation and western blot with other species antibody. And the sriking point is that I always find related genes to the one I am looking for, and transposons, always of the same kind, but not my gene. Do you have any idea of what is happening, does someone has a similar experience?
Thanks in advance!
Greetings!
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Postby JackBean » Mon Dec 10, 2012 6:13 pm

how much related are your related species? Are those antibodies against specific peptides or whole proteins?
I would suggest either partial purification of the protein if you're able to detect it in any way or gDNA/cDNA library and degenerated probe.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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