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About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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CFU conversion

Mon Nov 26, 2012 3:45 pm

How do I convert mol/CFU (colony forming unit) to millimol?

I came across a research paper saying:
The absolute amount of ATP per bacterium was calculated by dividing mol ATP ml-1 by c.f.u. ml-1.
There was a drop in ATP level (from 3.39±0.216 to 0.59±0.106 mol c.f.u.-1)

I want the ATP concentration (in bacterium) in millimol.
Will it be just 3.39+-0.216 x 10^3 millimol?
Or do I have to do some conversion for c.f.u?

How to do it?

Mon Nov 26, 2012 8:55 pm

you have to keep it in mmol/CFU

Tue Nov 27, 2012 12:54 am

The problem is, I want to use the concentration value somewhere else and I want it to be specified in millimoles.
Is it okay if I just ignore the cfu and use the value as millimol? Will it remain same? I just want the concentration of ATP inside the bacterial cell.

Tue Nov 27, 2012 10:09 am

Then it's technically per CFU, because each cell theoretically give rise to single colony.
On the other hand, the concentration inside cell will be different from that in media.

Mon Dec 03, 2012 8:22 am

Then I think I should just ignore the cfu and convert Mol to mMol, because it clearly specifies that the concentration is 3.39 mol/cfu.
Thanks a lot.
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