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Conversion of drug concentration from in vitro study to mice

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Conversion of drug concentration from in vitro study to mice

Postby MPBPhD » Fri Nov 16, 2012 10:27 pm

Can we estimate a dose (mg/kg) to be given to mice from the optimized concentration that elicits an effect in a cell-culture based system (nM)?

For example, if I empirically determined the optimal concentration of reagent ___ for an effect in ___ cell line is 100nM, how do I calculate the dose to be given to mice from it?

Converesly, if I know that the optimal nonseverly toxic dose of reagent ___ given to a mouse is 150 mg/kg, how do I calculate an equivalent concentration to be given to ___ cells for viability assays?

I plan to perform i.p. injections, although I am not sure if/how that would affect the answer.

Thank you for your answers!
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Postby JackBean » Wed Nov 21, 2012 6:16 pm

I don't think there is any easy conversion simply because the cells may not respond (and probably will not) in the same way as whole mouse.

Cis or trans? That's what matters.
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