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problem with migration assay!?!?!

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problem with migration assay!?!?!

Postby Halaqataruniversity » Thu Oct 18, 2012 9:13 am

Hello everyone :)

I'm doing migration assay lately, or some call it wound assay for HCT116 cells
anyhow, everything is going well with the scraching and so...
but when I see the cells under the microscope I usually find lots and lots of floating cells !!! its been annoying and it might affect my results somehow
hinse: I wash with PBS 2 times.
did anyone had this before?
I wish i can show u guys some picts i have but I don't know how to apload one here?

thanx
Halaqataruniversity
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