About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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I am working on a biology research project to identify a bacterial organism found in a small stream. Here is the info collected so far:
Colony morphology: Yellow, round, slightly concave, smooth.
Gram stain: Gram positive, appears to be cocci, but not perfectly round and tend to group together in pairs (which appear as though it is a short fat bacilli) or irregular clusters.
Blood Agar: Gamma hemolytic
EMB: Colorless growth (does not ferment lactose)
MS Agar: No growth
TSI: Yellow butt, yellow slant (ferments glucose, lactose and/or sucrose) (I am also wondering does this mean it does ferment lactose? Or can it mean that it ferments sucrose instead of lactose?)
Nitrate Reduction: Negative (does not break down NO)
Based on the first dichotomous key we had been using it led us to a bacteria called Gaffkya Tetragena, which I could not find any information on except for some very old medical journals (from the 30's and 50's). I found one page that mentioned something eluding to the fact that this species was renamed/reclassified to Aerococcus Viridans, which does not sound like it matches up with our organism. I looked at another bacterial flow chart and it led to micrococcus verians, and when I looked that up there also was not a lot of info, but mostly I found references to the fact that the micrococcus genus had been dissected into a new genus called Kocuria. I have also looked up this name, and cannot seem to find much information. If anyone has any advice on how I can find more info about this organism, tests we could try, or any other suggestions they would be much appreciated.
Hey your bacteria might be micrococcus luteus or something which is the same as micrococcus Kocuria, I believe. Try using the Bergy's manual of determinative bacteriology. Good luck and if you can take a look at mine and help me out that would be great!
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