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HELPP Please! Unidentifiable Unkown :(

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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HELPP Please! Unidentifiable Unkown :(

Postby ohyesitskim » Mon Oct 15, 2012 10:44 pm

I received an unknown in Microbiology glass and I just don't know what it is. Smell of broth is Pungent & rotten cheese. Gram Stain = Gram Positive. Arrangement: Strepto maybe staph? idk Shapes:Round ---Coccus. size: small (attached photo)
20120924_183641.jpg
my first stain
(my first stain)
20121003_181506.jpg
my later stain
(later stain, some may be pink because it was dying?)

Here are the test that i performed and their results.
Starch Plate -
Citrate Plate -
Phenol Red +
Gelatin Hydrolysis test not complete
SIM Medium -
Oxidation-Fermentation +
MR/VP +/ -
Oxidase +
Catalase +
EMB grew but my microbe is gram positive
20120926_185556.jpg
emb plate

1st side is mine 2nd side is E.coli

Phenylalanine Deaminase -
MIO -
Casein Hydrolysis (Milk Plate) -
ohyesitskim
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Still need help!

Postby ohyesitskim » Sun Oct 28, 2012 6:30 am

My lab report is due WEDS 10/28/2012. Please help.
Here are my overall test results. I know that it is streptococcus or enterococcus.

Test Results
Starch Plate -
Citrate Plate -
Phenol Red +
Gelatin Hydrolysis -
SIM Medium -
Oxidation-Fermentation +
MR/VP +/ -
Oxidase -
Catalase -
EMB inconclusive
Phenylalanine Deaminase -
MIO -
Bile Esculin +
Casein Hydrolysis (Milk Plate) -
Motility -
Wet Hang and Drop -




My options for what my bacteria is are:
Entercoccus facealis / streptococcus facealis
Lactococcus lactis/ streptococcus lactis
streptococcus mutans which i dont think it is because it isnt VP +
ohyesitskim
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