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Preparing cells for western blotting??

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Preparing cells for western blotting??

Postby Halaqataruniversity » Fri Sep 07, 2012 2:53 pm

Dear All,
i'm new in the cell culturing field and my Dr. asked me to prepare a western blotting protocol. its easy to understand each step, but im finding a diffeculty understanding the procedure in preparing cells before the protocol. what to do with the media n how to aspirate it and all... did some research but still no clear view!

I appreciate any siggestions...

Thnx :)
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Postby JackBean » Fri Sep 07, 2012 2:59 pm

cells? What exactly you wanna blot?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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