Join for Free!
121433 members

Preparing cells for western blotting??

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

Preparing cells for western blotting??

Postby Halaqataruniversity » Fri Sep 07, 2012 2:53 pm

Dear All,
i'm new in the cell culturing field and my Dr. asked me to prepare a western blotting protocol. its easy to understand each step, but im finding a diffeculty understanding the procedure in preparing cells before the protocol. what to do with the media n how to aspirate it and all... did some research but still no clear view!

I appreciate any siggestions...

Thnx :)
Posts: 3
Joined: Fri Sep 07, 2012 1:51 pm

Postby JackBean » Fri Sep 07, 2012 2:59 pm

cells? What exactly you wanna blot?

Cis or trans? That's what matters.
User avatar
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 3 guests