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Is protein degrade by UV ?

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Is protein degrade by UV ?

Postby afaaqb2w » Fri Aug 31, 2012 3:45 pm

Hi!!!
I,m doing a research in a protein extraction!! I just going to expose UV light to medium for the sterilization purposes.Will it work? and Is protein degrade by UV?
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Re: Is protein degrade by UV ?

Postby jonmoulton » Fri Aug 31, 2012 4:04 pm

It will depend on the wavelength of the UV light and its intensity. Aromatic amino acids, such as tyrosine, can absorb shorter-wavelength UV and become reactive. I found a lot of relevant information by searching the keyword group "UV tyrosine reactivity photochemistry" in Google -- try that.

If you are using UV to sterilize, you are trying to damage/kill microbes by messing with their proteins and nucleic acids. Yes, that can also mess with your analyte protein.
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Postby afaaqb2w » Sat Sep 01, 2012 7:19 pm

thank u for the replay..
U mean that microbes killed by UV by denaturing their protein also... I found some article say that UV denature the DNA therefore the microbes (mostly Bacteria) couldn't replicate..
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Postby JackBean » Mon Sep 03, 2012 8:39 am

Yeah, it will be bigger problems DNA damage for the microbes, because thymidine forms dimers etc.
However, proteins get damaged as well, they can get cross-linked by UV.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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