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microsatellite instability

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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microsatellite instability

Postby kk » Wed Aug 08, 2012 11:21 am

My question is what is the therapeutic advantage if someone with a colorectal tumor is diagnosed being microsatellite instable. Tx.
kk
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Postby kk » Mon Aug 13, 2012 1:32 pm

Actually I just came across one answer: "factors such as increased microsatellite instability have been associated with improved survival in patients with right-sided lesions."
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Postby kk » Tue Oct 08, 2013 9:47 am

Another question regarding MSI. When PCR amplifying microsatellite markers for subsequent fragment analysis by microcapillary elfo - is it important to use an ultra-high fidelity polymerase, or is it not important?
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Re: microsatellite instability

Postby kk » Thu Jul 10, 2014 2:45 pm

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?
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