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Restriction Digestion important

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Restriction Digestion important

Postby siddharthsameer » Wed Aug 01, 2012 4:17 pm

hello I need to ask one question and I am stuck with this kind;y help me...
The concentration of my pcr product is 33.56microgram/ml and I want to make the restriction digestion .I want to have the 2 microgram of my product in the ristriction digestion volume of 50microlitre. How much volume should i add of my pcr product to the reaction volume for the restriction...
kindly let me know I am stuckk and can not find the answer....please please help me
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Postby JackBean » Thu Aug 02, 2012 7:26 am

let's say it's 30 ng/ul and you want 2000 ng, how many-times is it? 2000/30 ~60-times -> thus you would need 60 ul
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