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Please Help Identify My Unkown Bacteria!!Moderator: BioTeam
3 posts • Page 1 of 1
Please Help Identify My Unkown Bacteria!!Hello All,
I am trying to identify my unkown bacteria for my Microbiology class. Below are the test result and information I have gathered thus far. Any possible guesses on Genus and/or species would be greatly appreciated. Initial Observation: opaque/ off white, slightly clump, slight growth on nutrient agar Presumed Optimum temp: 37C Oxygen Requiremets: Facultative Anerobe Bacillus shape (small rods) gram negative non spore forming not acid fast NEGATIVE for casein, starch hydrolysis Produces acid reaction with litmus milk Fermentaion of sucrose, glucose, lactose & mannitol. All with gas production Positive methyl red Neg Simmons Citrate (I did see a little blue *very minimal*, but still negative compared to the control) Neg indole Neg VP test Pos nitrate reduction ( produced nitrous acid) Pos TSI (hydrogen sulfide produced) Neg oxidase Pos catalase Neg Urea Possible bacteria I am considering, using Bergy Manual (but I could still be absolutely wrong): Genus Budvicia Genus Leminorella Genus Salmonella Genus Cedecea If any one has any possible ideas or can suggest an idea based on the addition of another test, let me know. Media is limited, so Im not sure whats available for me to use. Thanks KingsMomi
Re: Please Help Identify My Unkown Bacteria!!Where did you take your sample from and how was it collected?
I think it's either Pectobacterium wasabiae or Citrobacter freundii. Possibly Salmonella but not others you listed. Check the manual again. Also, minimal positive is positive not negative.
I suggest you check their characteristics and decide on the test that would distinguish between them. Good luck!
3 posts • Page 1 of 1
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