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bacterial culture growth

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bacterial culture growth

Postby siddharthsameer » Wed Jul 25, 2012 8:49 am

Hello everyone
I have one question , why do we measure the ph of Preculture.I made a a preculture of e.coli tof 10 for the competent cell, and i was asked to measure the ph of the preculture, and I had 3 precultre in different shake falsk, and the following pH I measured .
shake flask- pH
1 8,2
2 8,4
3 8,6
I dont know the reason behind it, kindly let me know... I am stuck and can not find the answer..I would be highly obliged to you all. waiting for your response.
with regards
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Postby canalon » Thu Jul 26, 2012 2:54 am

Never, ever did that. Sounds strange to me. Considering that they will be washed multiple times in plenty of different solutions and buffer any way...

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