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About Thiadiazuron(TDZ) and Phosphinothricin(PPT)

Plants!

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About Thiadiazuron(TDZ) and Phosphinothricin(PPT)

Postby yamini » Fri Sep 30, 2005 2:07 pm

Could working on plant tissueculture and genetic transformation answer my doubt.
I am trying to use TDZ to get transgenic shoots but my selection agent PPT seems to be sensitive. Even with very low PPT concentrations i am afailing to get shoots.
Can anyone help?

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Postby mothorc » Thu Oct 06, 2005 5:59 am

PPT in low content is a plant regulator, and there are some interaction between them but I can't find any article about this problem
If it is too sensitive, why don't you use another hormone to regenerate shoot. TDZ just a group of both auxin and cytokinin.
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