Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
sid: That's depends, whether is the PCR only to confirm that you have the gene cloned into vector or whether you want to do with the PCR amplicon something subsequently. I would bet on the first option, because pPICZ is expression vector.
Cis or trans? That's what matters.
I am about to start and my first step would be the amplification of my desired gene.. and then digestiona nd ligation into expression vector.. so If i start from the first step... thanks you so mucg for clearing my doubts...
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